Ezrin and HNRNP expression correlate with increased virus release rate and early onset of virus-induced apoptosis of MDCK suspension cells.
Identifieur interne : 000B84 ( Main/Exploration ); précédent : 000B83; suivant : 000B85Ezrin and HNRNP expression correlate with increased virus release rate and early onset of virus-induced apoptosis of MDCK suspension cells.
Auteurs : Sabine Kluge [Allemagne] ; Yvonne Genzel [Allemagne] ; Kim Laus [Allemagne] ; Anja Serve [Allemagne] ; Antje Pflugmacher [Allemagne] ; Britta Peschel [Allemagne] ; Erdmann Rapp [Allemagne] ; Udo Reichl [Allemagne]Source :
- Biotechnology journal [ 1860-7314 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Cellules rénales canines Madin-Darby, Chiens, Culture virale (), Libération de particules virales, Protéines du cytosquelette (métabolisme), Protéomique, Ribonucléoprotéines nucléaires hétérogènes (métabolisme), Sous-type H1N1 du virus de la grippe A (physiologie), Techniques de culture cellulaire.
- MESH :
- métabolisme : Protéines du cytosquelette, Ribonucléoprotéines nucléaires hétérogènes.
- physiologie : Sous-type H1N1 du virus de la grippe A.
- Animaux, Apoptose, Cellules rénales canines Madin-Darby, Chiens, Culture virale, Libération de particules virales, Protéomique, Techniques de culture cellulaire.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cytoskeletal Proteins, Heterogeneous-Nuclear Ribonucleoproteins.
- methods : Virus Cultivation.
- physiology : Influenza A Virus, H1N1 Subtype.
- Animals, Apoptosis, Cell Culture Techniques, Dogs, Madin Darby Canine Kidney Cells, Proteomics, Virus Release.
Abstract
With the aim to adapt high-yield adherent cell lines to suspension growth, Madin Darby canine kidney (MDCK) suspension cells were developed recently that achieved comparable influenza virus yields despite an early induction of apoptosis compared to the parental adherent cell line. For both cell lines, a comprehensive study under comparable infection conditions is performed comprising information on: time course of viral infection, antiviral state of cells, virus-induced apoptosis, and virus-induced cellular protein expression for early and late infection with influenza A/PuertoRico/8/34 H1N1. The proteomic analysis is performed with 2D differential gel electrophoreses followed by mass spectrometry. Based on flow cytometric data and on the differential expression of various stress and apoptosis-related proteins, the earlier onset of virus-induced apoptosis is confirmed for suspension cells. Surprisingly, the data indicated an increased virus release rate for suspension cells. These observations correlate with an increased expression of the apical marker protein ezrin, known to play a role in influenza-induced cytoskeletal rearrangement, and the differential expression of heterogeneous nuclear ribonucleoproteins, known to bind actively influenza viral proteins and play a central role in regulating gene expression. Based on these findings, additional studies towards the design of MDCK suspension cells with further increase in influenza virus yields will be performed.
DOI: 10.1002/biot.201600384
PubMed: 27581796
Affiliations:
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Le document en format XML
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<term>Apoptosis</term>
<term>Cell Culture Techniques</term>
<term>Cytoskeletal Proteins (metabolism)</term>
<term>Dogs</term>
<term>Heterogeneous-Nuclear Ribonucleoproteins (metabolism)</term>
<term>Influenza A Virus, H1N1 Subtype (physiology)</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Proteomics</term>
<term>Virus Cultivation (methods)</term>
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<term>Apoptose</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Culture virale ()</term>
<term>Libération de particules virales</term>
<term>Protéines du cytosquelette (métabolisme)</term>
<term>Protéomique</term>
<term>Ribonucléoprotéines nucléaires hétérogènes (métabolisme)</term>
<term>Sous-type H1N1 du virus de la grippe A (physiologie)</term>
<term>Techniques de culture cellulaire</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytoskeletal Proteins</term>
<term>Heterogeneous-Nuclear Ribonucleoproteins</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Virus Cultivation</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines du cytosquelette</term>
<term>Ribonucléoprotéines nucléaires hétérogènes</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
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<term>Apoptosis</term>
<term>Cell Culture Techniques</term>
<term>Dogs</term>
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<term>Apoptose</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Culture virale</term>
<term>Libération de particules virales</term>
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<front><div type="abstract" xml:lang="en">With the aim to adapt high-yield adherent cell lines to suspension growth, Madin Darby canine kidney (MDCK) suspension cells were developed recently that achieved comparable influenza virus yields despite an early induction of apoptosis compared to the parental adherent cell line. For both cell lines, a comprehensive study under comparable infection conditions is performed comprising information on: time course of viral infection, antiviral state of cells, virus-induced apoptosis, and virus-induced cellular protein expression for early and late infection with influenza A/PuertoRico/8/34 H1N1. The proteomic analysis is performed with 2D differential gel electrophoreses followed by mass spectrometry. Based on flow cytometric data and on the differential expression of various stress and apoptosis-related proteins, the earlier onset of virus-induced apoptosis is confirmed for suspension cells. Surprisingly, the data indicated an increased virus release rate for suspension cells. These observations correlate with an increased expression of the apical marker protein ezrin, known to play a role in influenza-induced cytoskeletal rearrangement, and the differential expression of heterogeneous nuclear ribonucleoproteins, known to bind actively influenza viral proteins and play a central role in regulating gene expression. Based on these findings, additional studies towards the design of MDCK suspension cells with further increase in influenza virus yields will be performed.</div>
</front>
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<name sortKey="Genzel, Yvonne" sort="Genzel, Yvonne" uniqKey="Genzel Y" first="Yvonne" last="Genzel">Yvonne Genzel</name>
<name sortKey="Laus, Kim" sort="Laus, Kim" uniqKey="Laus K" first="Kim" last="Laus">Kim Laus</name>
<name sortKey="Peschel, Britta" sort="Peschel, Britta" uniqKey="Peschel B" first="Britta" last="Peschel">Britta Peschel</name>
<name sortKey="Pflugmacher, Antje" sort="Pflugmacher, Antje" uniqKey="Pflugmacher A" first="Antje" last="Pflugmacher">Antje Pflugmacher</name>
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